处为100
Word资料
f30糖化所用时间，mi
1反应酶液的mL数六、注意事项1无论是标准液还是样品液，都要去除葡萄糖外的其他各种成分的对OD值的影响。使得到的标准曲线经过坐标原点。2用移液管或移液枪加各试剂时，不能将移液管或移液枪头混用。各比色管中，均用蒸馏水稀释至1mL，加35二硝基水酸显色剂3mL，在沸水浴中煮沸显色10mi
，冷却，加蒸馏水21mL，摇匀。
Determi
atio
ofCellulaseActivity
1PurposeofExperime
tMasterthepri
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Cellulaseca
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talI
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talSteps41Sta
dardCurveDrawi
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i
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Word资料
fwatershaki
gSetemptytubezerodetermi
eODvalueat550
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ateglucoseμg
umberasabscissatodrawthesta
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SerialNumber
1
2
3
4
5
6
glucosesta
dardsolutio

0
02
08
040060
100
mL
0
0
0
DistilledwatermL
1
08
02
060040
00
0
0
0
35di
itrosalir