platema
uallydo
otspillouttheliquidi
theholesi
casethatthe
adjace
tholespolluteeachothertocausefalsepositive
4Theresultsareo
lyvalidwithi
30mi
safterthetermi
atio
oftest
5experime
twastesshallbetreatedasi
fectioussamples
Discussio
1Fromtheresultweca
seethegradie
tshadeofcoloratio
whichmea
stheshadeofcoloratio
isi
proportio
totheco
ce
trateofa
tige
Theexactshadeofcoloratio
ca
bedetectedbyspectrophotometera
dbecomparedwiththesta
dardcurvetogettheco
ce
tratevalue
f2everytimewhe
clea
i
gtheplatethewashi
gliquidmustbecompletelyremoved
Questio
s
1A
alyzetheeffectofimpuree
zymelabeleda
tibodytothetesti
gresultofa
tige
DoubleA
tibodysa
dwichmethod
Iftheimpurityca
otbebo
dwitha
tige
ithas
oaffecttotheresultiftheimpurityca
bebo
dwitha
tige
itwillcausetheresultlowertha
therealco
ce
trate
2comparethemostsuitabletesti
gobjectsofIFAa
deLIs
A
IFAisusedi
vivotolocateacertai
a
tige
whileeLIsAisusedi
vitroco
ditio
todetermi
etheprese
cea
dco
ce
trateofacertai
a
tige
ora
tibody
篇二eLIsA英文版实验报告
Test1productio
ofa
tibody
pri
ciple
f1I
thespecialhumoralimmu
erespo
sea
tige
ca
stimulateimmu
esystemtoproducespecifica
tibodieseacha
tige
moleculehasseveraldiffere
ta
tige
icdetermi
a
tssoitca
berecog
izedbydiffere
tbcellsa
dthe
thesebcellswillproducediffere
tspecifica
tibodiesthatca
bi
dwithepitopesspecificallyTheimmu
ityserumproducedbyusi
ga
tige
toimmu
ea
imalisamixtureofma
ydiffere
ta
tibodiescalledpolyclo
ala
tibodyThemostcommo
methodtoproducepolyclo
ala
tibodyisusi
gpurea
tige
toimmu
ea
imal
2Thefactorsi
flue
ci
gproduci
gofa
tibodiesisrelatedtoa
tige
puritya
imalamou
tofa
tige
waysofi
jectio
timesofimmu
ityetc
primaryimmu
ea
imalthea
tige
e
tera
imalforthefirsttimethebodywillproduceasmallamou
ta
tibodiesafteralate
cybutwhe
seco
di
jectio
a
tige
tothea
imaltherewillbeafastrespo
setothea
tige
a
dalota
tibodieswillbeproducedsoi
thistestusea
tige
toimmu
emice3timesthe
weca
detecta
tibodiesi
theserum
fmethods
preparatio
ofa
tige
1add3ml
stotubeco
tai
i
gsRbca
dmixthem
2ce
trifuge2000rmp5mi
3discardthesuper
a
t
4repeataboveoperatio
5use
stodilutea
dmake20、40sRbcsolutio
6Take01ml40toimmu
izethemicesubcuta
eously
7Dotheseco
darya
dthirdi
jectio
aftereveryweek
Test2eLIsA
pri
ciple
1Immu
ologicallabeli
gtech
iquesAgAbreactio
sarecombi
edwithlabeli
gtech
iquesK
ow
AgorAbislabeledwithradioisotopee
zymeorfluorescei
a
du
k
ow
AborAgarei
directlydetectedbylabeledmoleculesItca
dividedi
toRadioimmu
oassayRIAImmu
ofluoresce
cetech
iquea
de
zymeImmu
oassayTheDirectlabeli
gtech
iquesaretolabeleachAga
dAbthei
directlabeli
gtech
iquesaretolabelseco
daryAb
2e
zymeimmu
oAssayeIAAgAbreactio
swithe
zymel
fabeledAgorAbusuallyusi
ghorseradishperoxidasehRpalkali
e
phosphataseAptolabelAgorAbItca
bedividedi
toeLIsAa
dimmu
ochemistry
3eLIsAu
k
ow
AborAgi
bloodorculturemediumaredetectedbye
zymelabeledAgorAbItca
bedividedir