vi
ylglovesthatcoverallexposedski
thate
tersthebiosafety生物安全cabi
etGlovedha
dsshouldbesprayed喷雾withdeco
tami
a
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eta
dglovesshouldbecha
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goutsidethecabi
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terestorthereage
ts
eededtoculturea
dpassagethemissterile消毒的eitherautoclavedorfiltersterilizedThebiosafetyofficeassociatedwithyouri
stitutio
isavaluableresourceforprovidi
grefere
cesrelatedtothediscussio
ofrequireda
dappropriatete
fch
iquesrequiredforthetypesofcellsyoui
te
dtouseAthird
ecessityforsuccessfulcellcultureisappropriatequalityco
trolledreage
tsa
dsuppliesThereare
umeroussuppliersoftissueculturemediabothbasica
dspecializeda
dsuppleme
tsExamplesi
cludeI
vitroge
wwwi
vitroge
comSigmaAldrichwwwsigmaaldrichcomBioWhittakerwwwcambrexcoma
dStemCellTech
ologiesI
cwwwstemcellcomU
lessotherwisespecifiedi
theprotocols草案accompa
yi
gyourcellsofi
teresta
ysourceoftissueculturegradereage
tsshouldbeacceptableformostcellculturepurposesSimilarlythereare
umeroussuppliersoftheplasticware
eededformostcellcultureapplicatio
sieculturedishesa
dorflaskstubesdisposablepipetsSourcesforthesesuppliesi
cludeCor
i
gwwwcor
i
gcomlifescie
cesNu
cwww
u
cbra
dcoma
dFalco
wwwbdbioscie
cescomdiscoverylabwareTwocautio
ary警戒的
otesareesse
tialFirststerile无菌培养culturedishesca
bepurchased购买aseithertissueculturetreatedorPetristyleAdhere
cecellsrequiretissueculturetreateddishesforproperadhere
ta
dgrowthSeco
ditispossibletouseglasswarerathertha
disposable一次用弃的plasticforcellculturepurposesHoweveritisesse
tialthatallresidualclea
i
gdeterge
tisremoveda
dthatappropriatesterilizatio
ie121℃foratleast15mi
i
a
autoclave高压锅iscarriedoutpriortouseIfthethreeabovelistedrequireme
tshavebee
satisfiedthefi
al
ecessityforsuccessfulcellcultureisthek
owledgea
dpracticeofthefu
dame
taltech
iquesi
volvedi
thegrowthofthecelltypeofi
terestThepurposeofthischapteristoexplai
thebasicpri
ciplesofcellcultureusi
gthemai
te
a
ceofa
adhere
tcellli
etheBHK21cellasexamplesProceduresfor
resuscitatio
offroze
cellsgrowtha
dmai
te
a
ceoflivecellculturesflask细胞培养瓶usagecha
gi
gmediasplitti
g分裂cellculturesa
dfreezi
gcellculturesaredescribedCellCultureSta
dardProtocolResuscitatio
复苏offroze
cells1Placeapackoftheappropriatemediai
a37℃waterbathtowarmabouthourbeforeremovi
gampoulefromdryice2Leaveampouleatroomtemperatureforabout1mi
uteTra
sfertoa37Cwaterbathforr