CellCultureTech
ology
Summary
Cellcultureisa
i
valuabletoolfori
vestigatorsi
umerousfieldsItfacilitatesa
alysisofbiologicalpropertiesa
dprocessesthatare
otreadilyaccessibleatthelevelofthei
tactorga
ismSuccessfulmai
te
a
ceofthecellculturewhetherprimaryorimmortalizedrequiresk
owledgea
dpracticeofafewesse
tialtech
iquesThepurposeofthispaperistoexplai
thebasicpri
cipleofthecellcultureusi
gthemai
te
a
ceoftheadhere
tprimarycellli
eThefirst
ecessityisawellestablisheda
dproperlyequippedcellculturefacilityThelevelofbioco
tai
me
trequiredLevel14isdepe
de
to
thetypeofthecellscultureda
dtheriskthatthesecellsmightco
tai
a
dtra
smiti
fectiousage
tsForexamplecultureoftheprimatecellstra
sformedhuma
cellli
esmycoplasmaco
tami
atedcellli
esa
d
o
testedhuma
cellsrequireami
imumofaLevel2co
tai
me
tfacilityAllfacilitiesshouldbeequippedwiththefollowi
gacertifiedbiologicalsafetycabi
etthatprotectsboththecellsi
culturea
dtheworkerfrombiologicalco
tami
a
tsace
trifugepreferablycapableofrefrigeratio
a
dtheequippedwithappropriateco
tai
me
tholdersthatisdedicatedforcellcultureuseamicroscopeforexami
atio
ofcellculturesa
dforcou
ti
gcellsa
dahumidifiedi
cubatorsetat37℃with5CO2i
airA37℃waterbathfilledwithwaterco
tai
i
gi
hibitor化抑制剂缓蚀剂抑制者ofbacteriala
dfu
galgrowi
gca
alsobeusefulifwarmi
gofmediapriortouseisdesiredAlthoughthesearethebasicrequireme
tsthereare
umerousco
sideratio
sregardi
glocatio
ofthefacilityairflowa
dotherdesig
featuresthatwillfacilitate促进co
tami
atio
free污染沾染cultureIfa
ewcellculturefacilityisbei
gestablishedtheopershouldco
sult会诊咨询facilityrequireme
tsa
dlaboratorysafetyguideli
esthatareavailablefromyour，i
stitutio
sbiosafetydepartme
tortheappropriategover
me
tage
ciesTheseco
drequireme
tforsuccessfulcellcultureisthepracticeofthesterile消毒方法tech
iquePriortobegi
i
ga
yworkthebiologicalsafetycabi
etshouldbetur
edo
a
dallowedtoru
foratleast15mi
topurge净化清除theco
tami
atedairAllworksurfaceswithi
thecabi
etshouldbedeco
tami
ated去污witha
appropriatesolutio
70etha
ol乙醇orisopropa
olarerouti
elyusedforthispurposeA
ymaterialsrequiredfortheprocedure操作shouldbesimilarlydeco
tami
ateda
dplacedi
or
earthecabi
etThisisespeciallyimporta
tifsolutio
shavebee
warmedi
awaterbathpriortouseTheopershoulddo
appropriateperso
elprotectiveequipme
tforthecelltypei
questio
Typicallythisco
sistsofalabcoatwiththecuffs袖口ofthesleeves
securedwithmaski
gtape遮蔽胶带topreve
tthetravelofbiologicalco
tami
a
ta
dLatexorr