油红O染色鉴定分化结果，可见细胞内大量脂滴；向成骨细胞诱导后，茜素红染色鉴定，肉眼可见阳性钙沉积。因此，五酶联合作用4h，在保证细胞高活性的前提下提高了细胞产量，缩短了原代细胞贴壁及融合时间缩短；培养获得的HUCMSC在增殖能力、表面标记及多向分化能力方面与传统方法相比无明显差别，为高效分离提取HUCMSC最优方案。结论该实验证明了
fⅡ型胶原酶、Ⅳ型胶原酶、胰蛋白酶、透明质酸酶和DNAase五酶联合消化脐带组织作用4h的方法缩短了分离细胞用时并显著提高了细胞产量、细胞活力以及细胞增殖能力，同时缩短原代细胞贴壁时间、融合时间。流式细胞仪检测细胞免疫学表型显示，该方法提取得到的原代细胞中MSCs比例，第三代MSCs细胞纯度较传统方法均有提高。因此，该方法是一种高效分离HUCMSCs的方法，可用来规模化提取培养HUCMSCs以满足科学实验、临床治疗的需求。
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