常用荧光染料的激发及发射波长
荧光素名称
激发波长发射波长备注
m
m
15IAEDANS
336
490
18ANS
372
480
4Methylumbellifero
e
385
502
5carboxy27dichlorofluorescei

504
529
5Carboxyfluorescei
5FAM
492
518
5Carboxy
apthofluorescei
pH10
512598563668RatioDyepH
5Carboxytetramethylrhodami
e5TAMRA542
568
5FAM5Carboxyfluorescei

492
518
5HATHydroxyTryptami
e
370415520540
5HydroxyTryptami
eHAT
370415520540
5ROXcarboxyXrhodami
e
578
604
567
591
5TAMRA
5Carboxytetramethylrhodami
e
548542
552568
6Carboxyrhodami
e6G
518
543
6CR6G
518
543
6JOE
520
548
7Ami
o4methylcoumari

351
430
7Ami
oacti
omyci
D7AAD
546
647
7Hydroxy4methylcoumari

360
449455
9Ami
o6chloro2methoxyacridi
e
412430471474
ABQ
344
445
AcidFuchsi

540
630
ACMA9Ami
o6chloro2methoxyacridi
e
412430471474
Acridi
eOra
geDNA
502
526
Acridi
eOra
geRNA
460
650
Acridi
eOra
gebothDNARNA
440480520650
Acridi
eRed
455600560680
Acridi
eYellow
470
550
Acriflavi

436
520
Acriflavi
Feulge
SITSA
355425460
Aequori
Photoprotei

466
Photoprotei

AFPsAutoFluoresce
tProtei
Qua
tum
Biotech
ologiesseesgGFPsgBFP
AlexaFluor350
346
442
342
441
AlexaFluor430
431
540
AlexaFluor488
495492519520
AlexaFluor532
531532553554
116
f常用荧光染料的激发及发射波长
AlexaFluor546AlexaFluor568AlexaFluor594AlexaFluor633AlexaFluor647AlexaFluor660AlexaFluor680
Alizari
Complexo
Alizari
RedAllophycocya
i
APCAMCAMCAS
AMCAAmi
omethylcoumari
AMCAXAmi
oacti
omyci
D
Ami
ocoumari
Ami
omethylcoumari
AMCA
A
ili
BlueA
throcylstearateAPCAllophycocya
i
APCCy7APTRABTCRatioDyeZ
2APTSAstrazo
Brillia
tRed4GAstrazo
Ora
geRAstrazo
Red6BAstrazo
Yellow7GLLAtabri
eATTOTAGCBQCAATTOTAGFQAurami
eAurophosphi
eGAurophosphi
eBAO9Bisami
ophe
yloxadiazoleBCECFhighpHBCECFlowpHBerberi
eSulphateBetaLactamaseBFPblueshiftedGFPY66H
556557572573
577578603
590594617618
632
650
647
666
668
698
679
702
530560580624
580
645
530560580
630645655660
345
445
345
425
347
444
353
442
555
655
346
442
350
445
345
425
347
444
600
360381446
630645655660
625650755
466380520530RatioDyeZ
2
424
505
500
585
470
540
520
595
450
480
436
490
465
560
486
591
460
550
450
580
450490515
365
395
492503520528
482
520
430
550
409
447520
381382445447blueshiftedGFP
216
f常用荧光染料的激发及发射波长
BlueFluoresce
tProtei
BFPGFPFRETBima
eBisbe
zamideBisbe
zimideHoechstbisBTCRatioDyeZ
2Bla
cophorFFGBla
cophorSVBOBO1BOBO3Bodipy492515Bodipy493503Bodipy500510Bodir