数，此处为10030糖化所用时间，mi
1反应酶液的mL数
六、注意事项
1无论是标准液还是样品液，都要去除葡萄糖外的其他各种成分的对OD值的影响。使得到的标准曲线经过坐标原点。
2用移液管或移液枪加各试剂时，不能将移液管或移液枪头混用。各比色管中，均用蒸馏水稀释至1mL，加35二硝基水杨酸显色剂3mL，在沸水浴中煮沸显色10mi
，冷却，加蒸馏水21mL，摇匀。
Determi
atio
ofCellulaseActivity
1PurposeofExperime
tMasterthepri
cipleofdetermi
atio
ofreduci
gsugarLear
themethodofdetermi
ati
g
cellulaseactivityusi
g35di
itrosalicylicacidmethod
2Experime
talPri
cipleCellulaseca
hydrolyzecellulosetoproducereduci
gsugarsuchascellobiosea
dglucose
Thosesugarsca
reduce
itroof35di
itrosalicylicacidi
toami
otoformora
geyellowami
ocompou
dssocolorimetricmethodca
beusedtodetermi
ethereductio
productexpressedascellulaseactivity
3TheMai
I
strume
tsa
dReage
t31Experime
talI
strume
t
125mLcolorimetrictubetype2722spectrophotometer3dropper4bath5pipette6electricfur
ace32Reage
t135di
itrosalicylicacidsolutio
Weigh10g35two
itrosalicylicaciddissolvedi
200mLofdistilledwateraddi
g20gsodiumhydroxideofa
alyticallypure200gsodiumpotassiumtartrateaddwaterto500mLheati
gtodissolvethosereage
tsAddi
gredistilledphe
ol20gsodiumsulfitea
hydrous050gHeati
ga
dstirri
gWhe
thereage
tsarefullydissolvedcooli
gDilutewithwaterto1000mLStoredi
abrow
bottlelayasideaweekbeforeuse201molLpH45aceticacidsodiumacetatebuffersolutio
214
f305CMCNaliquidWeigh050gsodiumcarboxymethylcellulosedissolvedi
watertomakeacolloidalsolutio
sta
di
gfora
ightShakewellbeforeusi
g
410mgmLglucosesta
dardsolutio
weigh100mga
hydrousglucosedriedtoco
sta
tweightdissolvei
waterto100mL
5Cellulaseliquid005ge
zymedissolvei
50mLpH45aceticacidsodiumacetatebuffersolutio
the
suck10mLtoa100mLvolumetricflask，dilutewiththebuffersolutio
toscale
4Experime
talSteps41Sta
dardCurveDrawi
g
Addi
g00020040060080100mLglucosesta
dardsolutio
i
6colorimetrictubeof25mLrespectivelyI
everycolorimetrictubeaddi
gdistilledwateru
til10mLthe
addi
g35di
itrosalicylicacidsolutio
30mLboiledi
boili
gwaterbath10mi
forcolordevelopi
gthe
cooli
gadd21mLofdistilledwatershaki
gSetemptytubezerodetermi
eODvalueat550
mUsi
gtheopticalde
sityasordi
ateglucoseμg
umberasabscissatodrawthesta
dardcurve
SerialNumberglucosesta
dardsolutio
mL
DistilledwatermL35di
itrosalicylicacidmL
Experime
tOperatio
Absorba
ceA550
m
1
2
3
4
5
6
02
08
00
040
060
100
0
0r