ALP定性染色(偶氮偶联法)
试剂:NephtholASMXPhosphateSigmaN4875NNDimethylformamideDMF二甲基甲酰胺DistilledWater02MTrisHClBufferpH85FastblueBBsaltSigmaF0250
ALP染液配方(即配即用,注意避光):总量试剂ASMXDMFddH2O02MTrisHClFBBsalt5ml05mg0025ml25ml25ml3mg10ml1mg005ml5ml5ml6mg50ml5mg025ml25ml25ml30mg
02MTrisHCl10MTrisHCl2mlddH2O8ml,调pH至85。使用时每孔加400μL现配的ALP染液,避光,置于摇床上,1h后观察。
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