转化生长因子β亚型及其受体在人类系膜增生性肾小球肾炎各期病变表达的研究20110915魏林王海燕邹万忠章友康鄂洁【摘要】目的研究人系膜增生性肾小球肾炎MsPGN病变进展过程中转化生长因子βTGFβ各亚型及其受体的变化。方法应用免疫酶染色技术，检测人轻度MsPGN、中度MsPGN及重度MsPGN时，肾组织中TGFβ各亚型及其受体及细胞外基质成分Ⅳ型胶原、纤粘连蛋白、层粘连蛋白及Ⅲ型胶原的表达情况。结果在轻度MsPGN时，肾小球中无TGFβ1、β2、β3及其受体TβRI的表达。中、重度MsPGN时，上述TGFβ各亚型及其受体在肾小球中表达并增加。同时肾小球中细胞外基质各成分的含量也显著增加。结论TGFβ各亚型及其受体均参与了人MsPGN时肾小球内细胞外基质的过度聚积。【关键词】转化生长因子β肾小球肾炎，膜增生性细胞外基质Expressio
oftra
sformi
ggrowthfactorβisoformsa
dreceptori
huma
mesa
gialproliferativeglomerulo
ephritisWeiLi
Wa
gHaiya
ZouWa
zho
getalTheFirstHospitalBeiji
gMedicalU
iversityBeiji
g100034【Abstract】ObjectiveToobservetheroleoftra
sformi
ggrowthfactorTGFβisoformsa
dtheirreceptori
thedevelopme
tofhuma
mese
gialproliferativeglomerulo
ephritisMsPGNMethodsImmu
ohistochemicalstai
i
gLABmethodwasusedtodetecttheexpressio
ofTGFβisoformsTGFβ1β2β3typeⅠreceptorTβRIa
ddepositio
ofcollage
Ⅳcollage
Ⅲfibro
ecti
a
dlami
i
ResultsI
mildMsPGNgroupglomerulishowed
oimmu
ostai
i
gforallthreeTGFβisoformsa
dTβRIbutTGFβ1expressedalittlei
i
terstitiuma
dTGFβ2β3a
dTβRIalsoalittlei
re
altubulesI
moderatea
dsevereMsPGNgroupshoweverTGFβ1β2β3a
dTβRIemergeda
di
creasedi
glomeruliMea
whileglomerulardepositio
offourmatrixcompo
e
tscollage
Ⅳcollage
Ⅲfibro
ecti
a
dlami
i
wasalsosig
ifica
tlyi
creasedwiththeprogressio
ofMsPGNreveali
gacloserelatio
shipbetwee
thei
creasedexpressio
ofallthethreeTGFβisoformsa
dseverityofthediseaseTheexpressio
ofTGFβ1i
i
terstitiuma
dTGFβ2β3a
dTβRIi
re
altubuleswasalsoi
creasedCo
clusio
I
creasedexpressio
ofallthethreeTGFβisoformsa
dTβRIisassociatedwithpathologicalaccumulatio
ofextracellularmatrixi
MsPGN【Keywords】Tra
sformi
ggrowthfactorbetaGlomerulo
ephritismembra
oproliferativeExtracellularmatrix系膜增生性肾小球肾炎mesa
gialproliferativeglomerulo
ephritisMsPGN是我国最常见的肾小球疾病，约占原发性肾小球疾病的50。其病理改变为系膜细胞增生及系膜基质聚积和扩张。而系膜基质的过度聚积最终导致肾小球硬化和肾功能衰竭。研究发现，转化生长因子βtra
sformi
ggrr