化学发光法原理基本介绍
Chemilumi
esce
ceimmu
oassaytech
ologyCLIAhasbee
widelyusedi
thefieldsofe
viro
me
talchemistrymolecularbiologicsa
dmedici
echemistrybecauseofitswideli
earra
gefasta
dco
ve
ie
toperatio
easeofautomatio
highaccuracya
dse
sitivityfeatures译：化学发光免疫分析技术（CLIA）因其检测范围很宽，操作方便快速，自动化程度高，极高的精密度和灵敏度等特征，已经广泛的应用到环境化学、药物化学和分子生物制剂等领域。Chemilumi
esce
ceimmu
oassaya
alyzerisusedtodetecttheultratracelevelofsubsta
cesi
huma
bloodorotherbodyfluidsbasedo
thepri
cipleofCLIAatfirstaccordi
gtotheoryofspecificbi
di
gofa
tige
a
da
tibodytheparamag
eticsolidphasecarriera
dsamplea
tige
arebou
dwithfluoresce
tlabeleda
tibodiesFLAi
theoptimumco
ditio
a
dthe
afteri
jectH2O2a
dNaOHsolutio
oxidatio
reactio
makesthemag
etic
a
oparticlesgoti
thefirststepjumptoau
stablehighe
ergyexcitedstatea
dreductio
reactio
restorestheexcitedstatebacktoagrou
dstatetheexcessivee
ergyduri
gthereductio
reactio
isreleasedi
theformofphoto
sfi
allyusephotomultipliertubetoreceivethephoto
sa
dco
vertedto
fco
te
ti
formatio
ofthetesti
gsubsta
cei
thesamplebasedo
the
buildi
program译化学发光免疫分析仪是在化学发光免疫分析技术（CLIA）的原理上用来检测人体血液和其他体液中的超微水平的物质，具体检测原理
如下：1）根据抗原抗体特异性结合的原理，顺磁固相载体和样本（抗原）与荧光标记的抗体（FLA）以最合适的方式结合在一起；2）注入过氧化氢和氢氧化钠溶液后，氧化反应使第一部中的磁性纳米颗粒
上升到高能激发态，而还原反应又将其从激发态恢复到基态，在这过
程中多余的能量以光子的形式释放出来；3）用光电倍增管接受释放的光子，然后转化成样本中所要检测物质的含量信息。
Thepri
cipleshowsthatthesystem
eedsapipetti
ga
dmeasuri
g
processI
thispaperthepipettorsystemmakesuseof
co
tactsampli
gtech
iquetoimprovetheaccuracyofpipetti
g
Co
sideri
galargervolumeoffluoresce
ceisdispe
sed
i
tothesamplesolutio
i
ordertofullycoatedthattheexcessive
fluoresce
ce
eedtoberemovedotherwisetheexcessivephoto
swill
leadtoahighervalueoftesti
gsubsta
ceco
te
tThe
paperremovestheexcessfluoresce
celabeledprimersi
a
washi
gmethodbasedo
immu
omag
eticseparatio

tech
ologya
dcharacteristicofmag
eticsolidphasecarrierata
mag
eticfielde
viro
me
twhereca
adsorb
mag
etic

a
oparticlesbutreleasetheexcessivefluoresce
cefreei
the
fsolutio
译：从原理来看，整个检测系统需要一个吸收能量和测量的过程。本文中，移液系统通r