．请每次测定的同时做标准曲线，最好做复孔。如标本中待测物质含量过高（样本OD值大于标准品孔第一孔的OD值），请先用样品稀释液稀释一定倍数（
倍）后再测定，计算时请最后乘以总稀释倍数（×
×5）。5．封板膜只限一次性使用，以避免交叉污染。6．底物请避光保存。7．严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准8．所有样品，洗涤液和各种废弃物都应按传染物处理。9．本试剂不同批号组分不得混用。10如与英文说明书有异，以英文说明书为准。
f计算：以标准物的浓度为横坐标，OD值为纵坐标，在坐标纸上绘出标准曲线，根据样品的OD值由标准曲线查出相应的浓度；再乘以稀释倍数；或用标准物的浓度与OD值计算出标准曲线的直线回归方程式，将样品的OD值代入方程式，计算出样品浓度，再乘以稀释倍数，即为样品的实际浓度。
试剂盒性能：1样品线性回归与预期浓度相关系数R值为095以上。2批内与批间应分别小于9和11
检测范围：5pgmL100pgmL
保存条件及有效期：1试剂盒保存：；28℃。2．有效期：6个月
fMouseI
terleuki
6
FORRESEARCHUSEONLY
DrugNames
Ge
ericName：MouseI
terleuki
6（IL6）ELISAKit
Purpose
Thiskitallowsforthedetermi
atio
ofIL6co
ce
tratio
si
Mouseserumbloodplasmaａ
dotherbiologicalfluids
Pri
cipleoftheassay
ThekitassayMouseIL6leveli
thesampleusePurifiedMouseIL6a
tibodytocoatmicrotiterplatewellsmakesolidphasea
tibodythe
addIL6towellsCombi
edIL6a
tibodywhichWithHRPlabeledbecomea
tibodya
tige
e
zymea
tibodycomplexafterwashi
gCompletelyAddTMBsubstratesolutio
TMBsubstratebecomesbluecolorAtHRPe
zymecatalyzedreactio
istermi
atedbytheadditio
ofasulphuricacidsolutio
ａ
dthecolorcha
geismeasuredspectrophotometricallyatawavele
gthof450
mTheco
ce
tratio
ofIL6i
thesamplesisthe
determi
edbycompari
gtheODofthesamplestothesta
dardcurve
fMaterialsprovidedwiththekit
MaterialsprovidedwiththekitUserma
ualClosureplatemembra
eSealedbagsMicroelisastripplateSta
dard：135pgmLSta
darddilue
tHRPCo
jugatereage
tSampledilue
tChromoge
Solutio
AChromoge
Solutio
BStopSolutio
washsolutio
48determi
atio
s121105ml×1bottle15ml×1bottle3ml×1bottle3ml×1bottle3ml×1bottle3ml×1bottle3ml×1bottle（20ml×20fold）×1bottle96determi
atio
s121105ml×1bottle15ml×1bottle6ml×1bottle6ml×1bottle6ml×1bottle6ml×1bottle6ml×1bottle（20ml×30fold）×1bottle28℃28℃28℃28℃28℃28℃28℃28℃28℃Storage
Specime
requireme
ts
1serumcoagulatio
atroomtemperature1020mi
s，ce
trifugatio
20mi
atthesper