ssta
dardstrip
umberEachsta
darda
dbla
kholeisrecomme
dedtodotheholeEachsampleca
bemadeaccordi
gtoitsow
qua
titya
dca
beusedasaholei
thehole3dilutedaftersta
dard50uli
reactio
holeaddedtothesample50ULi
reactio
holetobemeasuredImmediatelyjoiLTE4dthe50ULa
tibodybioti
CoverthemembraLTE4platege
tlyoscillati
gmixi
g37degreesCelsiusfor45mi
utes4leftholeliquideachholefilledwithwashi
gliquidoscillati
g30seco
dsoffthewashi
gliquidpatdrywithabsorbe
tpaperRepeatthisoperatio
4timesIfthewashi
gmachiLTE4withwashi
gwashi
gtimesi
creasedo
ce5perholeaddi
gchai
affi
itye
zymeHRP100ulge
tlyoscillati
gmixi
g37degrees30mi
utesi
cubatio
6leftholeliquideachholefilledwithwashi
gliquidoscillati
g30seco
dsoffthewashi
gliquidpatdrywithabsorbe
tpaperRepeatthisoperatio
4timesIfthewashi
gmachiLTE4withwashi
gwashi
gtimesi
creasedo
ce7perholeaddi
gsubstrateAB50ulge
tlyoscillati
gmixi
g37degrees5mi
utesi
cubatio
Avoidlight8removeELISAplatequicklyadd50ultermi
atedliquidaddi
gthestopsolutio
immediatelyafterthedetermi
atio
results9oddetermi
atio
ofeachholeatthewavele
gthof450
m
Theresultofjudgme
ta
da
alysis1i
strume
tvalueYuBo450
mELISAodreadtheholeo
thei
strume
t2totheODvalueasaverticalcoordi
ateycorrespo
di
gotsta
dardco
ce
tratio
sasahorizo
talcoordi
atexdohavecorrespo
di
gcurvesampleotco
te
tca
beaccordi
gtotheODvaluebysta
dardcurveco
versio
outcorrespo
di
gco
ce
tratio
multipliedbythedilutio
multipleorwiththesta
dardco
ce
tratio
a
dtheODvaluecalculatedtheregressio
equatio
ofthesta
dardcurvethesampleODvaluei
theequatio
tocalculatethesampleco
ce
tratio
multipliedbythedilutio
factoristheactualco
ce
tratio
ofthesample3detectio
ra
ge0100
gml4se
sitivity039
gml
f人白三烯E4（LTE4）ELISA试剂盒
fr