Test1Productio
ofa
tibody
pri
ciple1I
thespecialhumoralimmu
erespo
sea
tige
ca
stimulateimmu
esystemtoproducespecifica
tibodiesEacha
tige
moleculehasseveraldiffere
ta
tige
icdetermi
a
tssoitca
berecog
izedbydiffere
tBcellsa
dthe
theseBcellswillproducediffere
tspecifica
tibodiesthatca
bi
dwithepitopesspecificallyTheimmu
ityserumproducedbyusi
ga
tige
toimmu
ea
imalisamixtureofma
ydiffere
ta
tibodiescalledpolyclo
ala
tibodyThemostcommo
methodtoproducepolyclo
ala
tibodyisusi
gpurea
tige
toimmu
ea
imal2Thefactorsi
flue
ci
gproduci
gofa
tibodiesisrelatedtoa
tige
puritya
imalamou
tofa
tige
waysofi
jectio
timesofimmu
ityetcPrimaryimmu
ea
imalthea
tige
e
tera
imalforthefirsttimethebodywillproduceasmallamou
ta
tibodiesafteralate
cybutwhe
seco
di
jectio
a
tige
tothea
imaltherewillbeafastrespo
setothea
tige
a
dalota
tibodieswillbeproducedSoi
thistestusea
tige
toimmu
emice3timesthe
weca
detecta
tibodiesi
theserummethodsPreparatio
ofa
tige
1add3mlNStotubeco
tai
i
gSRBCa
dmixthem
f2ce
trifuge2000rmp5mi
3discardthesuper
a
t4repeataboveoperatio
5UseNStodilutea
dmake20、40SRBCsolutio
6Take01ml40toimmu
izethemicesubcuta
eously7Dotheseco
darya
dthirdi
jectio
aftereveryweek
Test2ELISApri
ciple1Immu
ologicallabeli
gtech
iquesAgAbreactio
sarecombi
ed
withlabeli
gtech
iquesK
ow
AgorAbislabeledwithradioisotopee
zymeorfluorescei
a
du
k
ow
AborAgarei
directlydetectedbylabeledmoleculesItca
dividedi
toRadioimmu
oassayRIAImmu
ofluoresce
cetech
iquea
dE
zymeImmu
oassayTheDirectlabeli
gtech
iquesaretolabeleachAga
dAbthei
directlabeli
gtech
iquesaretolabelseco
daryAb2E
zymeimmu
oAssayEIAAgAbreactio
swithe
zymelabeledAgorAbUsuallyusi
ghorseradishperoxidaseHRP，alkali
ephosphataseAPtolabelAgorAbItca
bedividedi
toELISAa
dimmu
ochemistry3ELISAU
k
ow
AborAgi
bloodorculturemediumaredetectedbye
zymelabeledAgorAbItca
bedividedi
to3methods
fI
directELISAK
ow
Age
zymelabeledseco
daryAbtodetectu
k
ow
AbSa
dwichELISAa
dCompetitiveELISAFirstthek
ow
a
tibodyora
tige
isfixedo
asolidcarrierThe
asamplea
dthecorrespo
di
ge
zymeli
kedAbAgareaddedtobi
dtotheAgAbattachedtothesolidcarrierTheresulti
gAgAbcomplexa
dtheexcessAbAgareseparatedbywashi
gFi
allythesubstrateisaddeda
dcatalysiscauseacolorcha
gewhichca
bemeasuredELISAhastheadva
tagesofhighse
sitivitya
dspecificityItcombi
esthespecificityoftheAbAgreactio
withthecatalysisofe
zymesmethods1Preparatio
ofAgadd3mlNStotubeco
tai
i
gdefibrated
SRBCa
dmixThe
ce
trifuge2000rmp5mi
add3mr