值荧光探针为了更好地在生物体系中实现对pH值变化实时、高灵敏度、原位和便捷地检测今后的相关研究工作可以集中在如下几个方面1开发双光子探针以降低生物自发荧光背景干扰进一步提高探针检测的灵敏度
2相比于开关型小分子荧光探针比值型荧光探针在检测时通过荧光光谱图两个荧光峰强度
f变化对同一物质进行检测使其具备了内在的自校准功能因而在生物体系中对于pH值的定量检测结果更为准确和可靠因此发展有机小分子pH值荧光探针仍然是未来研究的一个重要方向3开发出稳定的近红外pH值荧光探针有利于穿透更深的生物组织从而可以实现
活体内微环境的pH值变化检测4发展基于小分子探针的pH值传感器并将其与手机应用软件结合制作出可穿戴式的pH值设备实现对人体等生物体系中微环境pH值的快速动态
检测
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