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免疫透射比浊法与散射比浊法检测血清胱抑素C的方法学比较
作者：刘雯龚玲曹伟俞莹应筱雯鲍彩丽杜山青来源：《中国现代医生》2017年第21期
摘要目的对免疫透射比浊法测定血清胱抑素C的方法学性能行初步评价，并与免疫散射比浊法进行比较。方法应用RoheModularP全自动生化分析仪与SIEMENSBNProSpec特定蛋白仪分别对血清胱抑素C进行初步的方法学评价，包括精密度、线性回归试验、回收试验、干扰试验。结果免疫透射比浊法和免疫散射比浊法无论是批内还是批间精密度均符合要求，CV均095；免疫透射比浊法与免疫散射比浊法的回收率均96；干扰物试验结果显示血红蛋白、三酰甘油及胆红素均对测定无明显影响。结论免疫透射检验的灵敏度较高，检测范围广，与免疫散射试验的结果基本一致，系统误差小、抗干扰能力强，且更加快速、实用和便利。关键词免疫透射比浊法；免疫散射比浊法；血清胱抑素C；方法学；检验中图分类号R4466文献标识码B文章编号16739701（2017）21009603Methodologicalcompariso
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C；Methodology；Test胱抑素C（CysC）又称为半胱氨酸蛋白酶抑制剂C。近年来，由于高血压、糖尿病发病率r